Posted September 27, 2022

Research Engineer Position available: "Anti-tumor immunity by targeting the extracellular matrix with CAR T cells".

In the INCa PLBIO funded project “Matrix-CAR-Targeting”: anti-tumor immunity by targeting the extracellular matrix with CAR T cells" three research laboratories (E. Donnadieu, Institut Cochin, Paris, C. Ferrand, EFS, (French Blood Center), Besançon, G. Orend, INSERM U1109, Strasbourg) are collaborating in developing and applying novel tools to target the extracellular matrix for reprogramming the immune suppressive tumor microenvironment including peptides and matrix CAR (chimeric antigen receptor) specific CAR-T cells. Spatial distribution, migration and activation of engineered T cells will be investigated in spheroid cultures, ex vivo tumor tissue slices, and cancer progression models. Tumor immunity, growth, lung metastasis and tumor microenvironment properties will be determined by flow cytometry, tissue staining, cytokine array and RNA seq analysis.

A 36 months position (research engineer) starting in spring 2023 is available in the Tumor Microenvironment group of Gertraud Orend (INSERM U1109, Strasbourg). This laboratory (https://orend-tme-group.com) is specialized in the analysis of the tumor microenvironment with particular emphasis on the extracellular matrix molecule tenascin-C (Yimaz et al., 2022, J Cell Sci, Midwood et al., 2016, J Cell Sci). This laboratory has demonstrated pivotal roles of tenascin-C in tumor angiogenesis (Saupe et al., 2013, Cell Reports, Rupp et al., 2016, Cell Reports), metastasis (Sun et al., 2018, Cancer Res, Sun et al., 2019, Mat Bio) and tumor immunity (Deligne 2020, Cancer Immun Res, Spenle et al., 2021, Front Immunol), and recently showed that tenascin-C orchestrates an immune suppressive tumor microenvironment. The novel concept of "TIL-Matrix Retention" where tenascin-C immobilizes tumor infiltrating leukocytes (TIL) could explain how matrix counteracts immune checkpoint therapy (Spenlé et al., 2020, Cancer Immun Res, Murdamoothoo et al., 2021 EMBO Mol Med). In frame of this project the candidate will apply novel CAR T cells and the recently published MAREMO peptides (Loustau et al., 2022, Mat Bio).

We offer: a highly dynamic and supportive group of colleagues including researchers, postdocs, PhD and master students and technical personnel with expertise in extracellular matrix research, murine tumor models and tumor immunity. The salary remuneration follows INSERM guidelines taking into account previous experience.

We search: a highly motivated scientist with strong background in tumor biology, mouse tumor models, immunology and cell culture, high team spirit and good English communication skills.

Interested candidates are invited to send their CV together with a motivation letter and the names of three referees to Gertraud Orend (gertraud.orend@inserm.fr)

 

Posted September 13, 2022

Two postdoctoral positions in cardiovascular research

The research group Cardiovascular research - Translational studies at Lund University is looking for two senior postdocs, highly-skilled in experimental techniques to help validate novel targets and mechanisms in our unique human biobank. If you are motivated to become part of a very international team at the Clinical Research Center, Lund University and fight against atherosclerosis, please do contact us!
Find out more about the positions on Varbi: https://lnkd.in/d2ZbCv4y
Contact: Isabel Gonçalves, MD, PhD, FESC, Professor/Senior Consultant Cardiology, Skåne University Hospital & Clinical Sciences Malmö, Lund University, Sweden
email: isabel.goncalves@med.lu.se

Posted September 9, 2022

Impact of the extracellular matrix architecture on metastasis formation

 

We work on the impact of the extracellular matrix architecture on metastasis formation. The extracellular matrix is as fascinating as complex and presents the house cells build, live in, move through, and constantly repair and remodel. We are a relatively young group with a strong passion to decode the physico-chemical properties of the extracellular matrix in vitro, ex vivo, and in vivo during metastasis formation. We do not only focus on basic aspects of the protein mass within the intercellular space but also on translating findings into the clinic. The position will be in close contact with the Faculty of Medicine in Freiburg and with several internationally collaboration partners. This work includes a multitude of techniques spanning biomechanical analysis in combination with multi-omics approaches, patient-derived organoids, animal work, biochemistry, and bioinformatic tools.

We are looking for:

  • university degree in the following areas but not essentially restricted to: biology, chemistry, physics, engineering, pharmaceutical science, informatics

  • English language skills

  • eager to discover underlying mechanisms of living systems, passionate and enthusiastic for Science

  • bent to perform impactful research

 

For more information, send an email to:
florin-andrei.taran@uniklinik-freiburg.de
raphael.reuten@pharmakol.uni-freiburg.de

Please apply online before November 15th, 2022, at this link

Posted September 9, 2022

Structure-function analysis of a prominent target in fibrosis: the C-terminal maturation complex of fibrillar collagens

The biosynthesis of fibrillar collagens is severely deregulated in fibrosis, the common and deleterious outcome of a great diversity of tissue injuries. A better understanding of the various steps leading from the synthesis of individual procollagen chains to collagen fibrils would greatly benefit the development of new therapeutic tools. In the P3-complex project, we focus on the C-terminal proteolytic maturation of fibrillar procollagens in order to:
- better understand, at the molecular level, how the various components of the maturation complex (substrate, protease, regulatory proteins) associate and work together. This is done mainly by cryo-electron microscopy and through a combination of biochemical/biophysical approaches.
- learn more about how this complex affects tissue biology and extracellular matrix remodelling using transcriptomics, targeted mass spectrometry and biochemical assays.
This project is funded by the French Research Agency (ANR) and is performed in collaboration with the OPIC (Oxford Particle Imaging Center) facility in Oxford for cryo-EM and with the University Hospital of Dijon for fibrosis samples. It will contribute to optimize innovative tools, currently under development in the group, to diagnose and treat fibrosis.

Skills : 
- Excellent theoritical and practical knowledge of molecular biology and protein biochemistry
- Experience in running protein-protein interaction analyses (ideally SPR) and biochemical assays to measure protein concentrations (ELISA, targeted mass spectrometry)
- Knowledge in structural biology; previous experience in cryo-EM would be an advantage
- Knowledge in extracellular matrix biology

More details here. Download the flyer.

We invite highly motivated candidates to apply directly on the above website (detailed CV with publication list and names of 2 references, cover letter) before October 15, 2022. The position is already open and can start immediately.
 

Laboratoire de Biologie Tissulaire et Ingénierie thérapeutique
Institut de Biologie et Chimie des Protéines
Unité Mixte de Recherche 5305 - CNRS / Université Lyon 1
https://lbti.ibcp.fr/

Posted July 30, 2022

Openings at all levels in the Naba lab at the University of Illinois at Chicago

The Naba lab at the University of Illinois at Chicago studies the role of the extracellular matrix (ECM) in development, health, and disease, with a particular focus on cancer. To do so, we utilize classical molecular, cellular, and developmental biology approaches in combination with cutting-edge proteomics and computational analyses. Our goal is to better understand how the ECM contributes to diseases so that we can exploit it to develop novel diagnostic and therapeutic strategies.

The Naba lab is a highly collaborative and dynamic group that strives for scientific excellence and offers a stimulating and inclusive working environment conducive to learning and professional development. 

The Naba lab is seeking highly motivated candidates to fill multiple positions at pre-doctoral and post-doctoral levels, read here for more information: https://sites.google.com/a/uic.edu/nabalab/join-us

Posted May 29, 2022

Postdoc position: collagen trafficking and multicellular communication and coordination to maintain collagen homeostasis

The newly established Chang lab is looking for a motivated person to join as a Postdoctoral research associate, to undertake a new direction to study collagen trafficking, and how different cell types communicate and coordinate to maintain collagen homeostasis. This project will involve techniques such as mass spectrometry, flow cytometry, genome editing, and mouse models. There will also be clinical links, in particular to lung fibrosis and chronic skin wounds.

A job description/person specification (start date is actually 1st of July, not June) and the website for application is https://www.jobs.manchester.ac.uk/displayjob.aspx?isPreview=Yes&jobid=22390.

Any enquiries could be directed to joan.chang@manchester.ac.uk. Application deadline is 6th of June

Posted November 5, 2021

Postdoc position: Tumor stroma and extracellular matrix as a modulator of immunotherapy efficacy, CCIT-DK, Copenhagen University Hospital, Herlev, Denmark.

The "Tumor Stroma and Matrix Immunology" (TSMI) group headed by Associate Professor and Lundbeck Fellow, Daniel H. Madsen at the National Center for Cancer Immunotherapy (CCIT-DK), Copenhagen University Hospital, Denmark, is seeking a highly motivated candidate for a 3-years postdoc position (February 1st, 2022 or after agreement) within the field of cancer immunology/matrix biology. The applicant must have a background in cancer biology, matrix biology, or immunology. Prior in vivo research expertise with mouse work is an advantage.

For more information and to submit your application, please visit this website: link

Posted September 30, 2021

Two-year fixed-term contract signaling in Oncogenesis, Angiogenesis and Permeability, Cancer & Immunology Research Center, INSERM, CNRS, Nantes University.

The “Signaling in Oncogenesis, Angiogenesis and Permeability” (SOAP) team is interested in deciphering how tumor cells pirate basic signaling pathways to sustain their survival and unlimited proliferation, as well as the way in which they interact within their environment. Fundamental signaling mechanisms are explored with a specific emphasis on deleterious remodeling of the vascular network associated to tumor. We developed a specific expertise in the characterization of intracellular signaling pathways with a focus on brain tumors. More specifically, we have identified key factors released in the tumor microenvironment involved in tumor/endothelial bidirectional interactions. How this is translated into endothelial plasticity in the course of tumor progression warrants further investigation. Our project combines high throughput unbiased screens (proteomic, genomic and chemical) with state-of-the-art biochemistry and cell biology (2D/3D cell models, super-resolution), as well as integrated models (mouse models and clinical samples) to explore intracellular signaling and cell communication. We anticipate that our results will increase our knowledge on basic signaling mechanisms involved in tumor initiation, progression and resistance, and may help the design of new strategies to face devastating human cancers.

For more information visit this link.

Posted March 27, 2021

One postdoctoral fellowships in Dr. Van Obberghen-Schilling's Lab, Institute of Biology Valrose, Université Côte d’Azur (UCA).

3IA Côte d’Azur Research Axis : AI for Computational Biology and Bio-Inspired AI

 

Applications are invited for a 2-year 3iA Côte d’Azur postdoctoral position in tumor/ECM biology to study functional and structural features of the extracellular matrix (ECM) in the immunosuppressive tumor microenvironment of head and neck cancer. Immunomodulatory therapies are promising for this tumor type, yet resistance rates are high since less than 20% of patients respond. We are specifically interested in exploring the tumor ECM environment, together with immune cell signatures, for gaining mechanistic insights into invasive disease and resistance to immunotherapy. Our previous work on ECM topology based on confocal images of cell-derived ECM has provided a framework for quantitative description and modeling of matrix features associated with disease states. The present project involves quantitative characterization of ECM architecture in in vitro models and human tumor tissue using multiplex immunofluorescence imaging, empowered by deep learning approaches.
For more information visit this link.